Alzmap
Alzmap is a data website
accompanying our "Spatial transcriptomics and in situ sequencing
uncover coordinated cell responses to amyloid-β plaques in
Alzheimer’s disease" paper, created at the
Bart
de Strooper laboratory for research of neurodegenerative
diseases in Leuven, Belgium.
Summary
While complex inflammatory-like alterations are observed around
the amyloid plaques of Alzheimer disease (AD), little is known
about the molecular changes and cellular interactions that
characterize this response. We investigate here in an AD mouse
model the transcriptional changes occurring in tissue domains of
100 μm diameter around the amyloid plaques using spatial
transcriptomics. We demonstrate early alterations in a gene
co-expression network enriched for myelin and oligodendrocyte
genes (OLIG), while a multicellular gene coexpression network of
Plaque-Induced Genes (PIGs) involving the complement system,
oxidative stress, lysosomes and inflammation is prominent in the
later phase of the disease. We confirm the majority of the
observed alterations at the cellular level using in situ
sequencing on mouse and human brain sections. Genome-wide spatial
transcriptomic analysis provides an unprecedented approach to
untangle the dysregulated cellular network in the vicinity of
pathogenic hallmarks of AD and other brain diseases.
